Research article
Local administration of glucocorticoids decreases synovial citrullination in rheumatoid arthritis
1 Department of Medicine, Rheumatology Unit, Karolinska University Hospital, Karolinska Institutet, SE- 141 86, Stockholm, Sweden
2 University of Alabama at Birmingham and Birmingham Veterans Administration Medical Center, S. 19th Street, Birmingham, AL 35233, USA
3 Department of Biomolecular Chemistry, Radboud University Nijmegen, PO Box 9102, 6500 HC, Nijmegen, The Netherlands
Arthritis Research & Therapy 2012, 14:R20 doi:10.1186/ar3702
Published: 27 January 2012Additional files
Additional file 1:
Methotrexate (MTX) has no effect on expression of citrullinated proteins and PAD4 and PAD2 in SFMCs. Brown diaminobenzidine immunoperoxidase staining detects citrullinated proteins, as detected with F95 antibody, PAD2 as detected with ROI001 antibody, and PAD4 as detected with SN823 antibody. Original magnification, ×200. Graphs show results of manual counting in six different SFMC samples analyzed in duplicate, and results are expressed as fold decrease of the number of positive cells in the treated samples as compared with controls.
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Additional file 2:
MTX has no effect on expression of citrullinated proteins and PAD4 expression in an RA synovial explant. Brown diaminobenzidine immunoperoxidase staining shows citrullinated proteins as detected with F95 antibody, PAD2 as detected with ROI001 antibody, and PAD4 as detected with SN823 antibody. Original magnification, ×250.
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