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Decreased influenza-specific B cell responses in rheumatoid arthritis patients treated with anti-tumor necrosis factor

James J Kobie1, Bo Zheng1, Peter Bryk1, Michael Barnes2, Christopher T Ritchlin1, Darren A Tabechian1, Allen P Anandarajah1, R John Looney1, Ralf G Thiele1, Jennifer H Anolik1, Andreea Coca1, Chungwen Wei1, Alexander F Rosenberg1, Changyong Feng3, John J Treanor4, F Eun-Hyung Lee2 and Ignacio Sanz1*

Author Affiliations

1 Division of Allergy, Immunology and Rheumatology, University of Rochester Medical Center, 601 Elmwood Avenue, Box 695, Rochester, NY, 14642, USA

2 Division of Pulmonary & Critical Care Medicine, University of Rochester Medical Center, 601 Elmwood Avenue, Box 692, Rochester, NY, 14642, USA

3 Department of Biostatistics and Computational Biology, University of Rochester Medical Center, 601 Elmwood Avenue, Box 630, Rochester, NY, 14642, USA

4 Division of Infectious Disease, University of Rochester Medical Center, 601 Elmwood Avenue, Box 689, Rochester, NY, 14642, USA

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Arthritis Research & Therapy 2011, 13:R209  doi:10.1186/ar3542

Published: 16 December 2011

Additional files

Additional file 1:

Additional Table 1. HAI seroprotection proportion. Peripheral blood was obtained prior to (baseline), and one and six months following vaccination with trivalent influenza vaccine (TIV) throughout multiple years. Influenza-specific serum antibody was measured by hemagglutination inhibition assay (HAI) for the indicated influenza type. The number of subjects with HAI titer of 40 or higher/total number of subjects (%) are indicated. Relative difference in geometric mean titer (GMT) among rheumatoid arthritis (RA) groups as compared with the healthy control (HC) group at same time point indicated by color. Cells with bold outline indicate significant difference (P < 0.05) as compare with the HC group at same timepoint.

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Additional file 2:

Additional Figure 1. TIV-specific IgG memory B cell frequency. Total B cells were isolated at baseline and one and six months following immunization with 2006/2007 through 2009/2010 trivalent influenza vaccine (TIV), cultured with CpG and IL-2 for four days, and TIV and total IgG specific EliSpots performed to determine the frequency of TIV specific memory B cells. An individual subject may have provided data from multiple study years. No statistical difference (P < 0.05) was detected among groups at individual time points.

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