CD20+, IL-17-secreting, and IL-17-secreting/CD20+ lymphocytes in healthy subjects (HS) and rheumatoid arthritis (RA) patients. (A) Dot plot of size versus CD20 positivity in representative peripheral blood lymphocyte (PBL) samples from an HS and an RA patient. (B) Typical flow cytometry dot plots showing the proportion of CD20+ and IL-17-secreting lymphocytes in representative PBL samples from an HS (left panel) and an RA patient (right panel). Each dot plot shows the proportion of CD20+ cells (lower right quadrant), IL-17-secreting cells (upper left quadrant), and IL-17-secreting CD20+ cells (upper right quadrant). (C) The proportion of PBLs that were IL-17-secreting in HS (n = 6) and RA subjects (n = 9, left panel) and the proportion of IL-17-secreting cells that were specifically CD20+ T cells (right panel). The horizontal bars represent the median percentage and interquartile range of each group. IL, interleukin.
Eggleton et al. Arthritis Research & Therapy 2011 13:R208 doi:10.1186/ar3541