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Open Access Highly Accessed Research article

Follistatin-like protein 1: a serum biochemical marker reflecting the severity of joint damage in patients with osteoarthritis

Yuji Wang12*, Dawei Li2*, Nanwei Xu1, Weijian Tao3, Ruixia Zhu1, Rongbin Sun1, Weiwei Fan2, Ping Zhang4, Tianhua Dong5 and Long Yu2

Author Affiliations

1 Department of Orthopaedics, Changzhou No.2 People's Hospital, the Affiliated Hospital of Nanjing Medical University, 29 Xinglong Alley, Changzhou, 213003, P. R. China

2 State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, 220 Handan Road, Shanghai, P. R China

3 Department of Orthopaedics, Suzhou Xiangcheng People's Hospital, 1060 Huayuan Road, Suzhou, 215131, P. R. China

4 Clinical laboratory, Changzhou No.2 People's Hospital, the Affiliated Hospital of Nanjing Medical University, 29 Xinglong Alley, Changzhou, 213003, P. R. China

5 Department of Orthopaedics, the First Affiliated Hospital of Suzhou University, 188 Shizi Street, Suzhou, 215006, P. R. China

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Arthritis Research & Therapy 2011, 13:R193  doi:10.1186/ar3522

Published: 25 November 2011

Abstract

Introduction

Follistatin-like protein 1 (FSTL1) is a secreted glycoprotein that has been implicated in arthritis pathogenesis in a mouse model. The aim of this study is to detect FSTL1 expression and to further assess its potential utility as a biomarker of joint damage in osteoarthritis (OA) patients.

Methods

FSTL1 expression was detected by real-time PCR, western blot and immunohistochemistry (IHC) in the synovial tissues (STs) and by IHC in the articular cartilage from OA patients and control trauma patients. The serum and synovial fluid (SF) FSTL1 concentrations were measured by ELISA in OA patients and control individuals. Linear regression analyses were used to assess correlations between the serum FSTL1 levels and the clinical characteristics in OA patients.

Results

The FSTL1 mRNA and protein levels were substantially elevated in the STs from OA patients compared with those from control trauma patients. The FSTL1 expression was strong in the cytoplasm of the synovial and capillary endothelial cells of the STs, but weak in the chondrocytes of the articular cartilage from OA patients. Furthermore, the serum and SF FSTL1 concentrations were significantly higher in OA patients than in respective control subjects. Interestingly, the serum and SF FSTL1 levels were markedly higher in female OA patients than in males. Importantly, bivariate regression analysis revealed that the serum FSTL1 levels in female OA patients had significant correlations with Kellgren and Lawrence (KL) grade, joint space narrowing (JSN) and the Western Ontario McMaster and Universities Osteoarthritis (WOMAC) stiffness subscale, an inverse correlation with height, and marginal correlations with the total WOMAC score and the WOMAC function subscale. Multivariate regression analysis revealed that the serum FSTL1 levels correlated independently with KL grade in female OA patients. Bivariate analysis also revealed that the serum FSTL1 levels correlated significantly with age and disease duration, and they correlated marginally with high sensitivity C-reactive protein (hs-CRP) and KL grade in male OA patients.

Conclusions

Increased FSTL1 expression may be a characteristic of OA patients. FSTL1 is a potential serum biomarker that may reflect the severity of joint damage, and further studies are required to evaluate its potential application for monitoring the course of the disease and the efficacy of therapies in OA patients.

Keywords:
FSTL1; osteoarthritis; biochemical marker; KL grade; WOMAC score