Production of IL-6 by peripheral monocytes stimulated with IFN-γ. Monocytes (2.5 × 105/ml) prepared from normal individuals (Nr) (open column) and primary Sjögren's syndrome (pSS) patients (checkerboard column) were cultured for 96 hours without stimulation. pSS monocytes were similarly cultured in the presence of 200 ng/ml of recombinant human IFN-γ (closed, hatched and gray columns), and simultaneously exposed to none (closed column), an anti-BAFF antibody (10 μg/ml; hatched column) or a control IgG (10 μg/ml; gray column). The amounts of IL-6 in the culture supernatants were measured by sandwich ELISA. BAFF = B-cell-activating factor of the TNF family; IgG = immunoglobulin G. Data represent means ± SEM. *P < 0.05 and **P < 0.01.
Yoshimoto et al. Arthritis Research & Therapy 2011 13:R170 doi:10.1186/ar3493