Table 1

Primers sets for the truncation of HCMVpp65 antigen constructions

Clones

Sequences

Vectors


Forward (5- > 3)

Reverse (5- > 3)


pp651-167

AT

    G GAT CC
A TGG AGT CGC GCG GTC GCC G

CCG

    GAA TTC
CAG TCC CGA GAC CGT GAG GAC CGT

pET30

pp65167-336

CGC

    GGA TCC
TGG ACG CGT CAG CAG ACC CA

CGC

    GGA TCC
CTC GCG TAT GGC TTG TAC CT

pET30

pp65336-561

CGC

    GGA TCC
ACC GTG GAA CTG CGT CAG TA

TA

    G GAT CC
A CCT CGG TGC TTT TTG GGC G

pET30

pp65336-448

CGC

    GGA TCC
ACC GTG GAA CTG CGT CAG TA

CGC

    CTC GAG
CGA CGT GCA CGC CGT CGC

pET30

pp65336-439

CGC

    GGA TCC
ACC GTG GAA CTG CGT CAG TA

CGC

    CTC GAG
TGA TTT GCG TTT GCG GCC

pET30

pp65336-422

CGC

    GGA TCC
ACC GTG GAA CTG CGT CAG TA

CGC

    CTC GAG
GCC GGTGAC GCG GGG CGT

pET30

murine C3d

CGC

    GAT AT
C ACC CCC GCA GGC TGT GGG GAA C 3'

CGC

    GGA TCC
GGA TCC GCT ACG GCT GGG GAG

pET32

pp65336-439

CGC

    GGA TCC
ACC GTG GAA CTG CGT CAG TA

CGC

    CTC GAG
TGA TTT GCG TTT GCG GCC

pAS-1


The underlined sequences mean the usage of restrictive enzyme

Hsieh et al. Arthritis Research & Therapy 2011 13:R162   doi:10.1186/ar3481

Open Data