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Table 1
Primers sets for the truncation of HCMVpp65 antigen constructions
Clones	Sequences		Vectors

	Forward (5- > 3)	Reverse (5- > 3)

pp65_1-167	ATG GAT CCA TGG AGT CGC GCG GTC GCC G	CCG GAA TTC CAG TCC CGA GAC CGT GAG GAC CGT	pET30
pp65_167-336	CGC GGA TCC TGG ACG CGT CAG CAG ACC CA	CGC GGA TCC CTC GCG TAT GGC TTG TAC CT	pET30
pp65_336-561	CGC GGA TCC ACC GTG GAA CTG CGT CAG TA	TAG GAT CCA CCT CGG TGC TTT TTG GGC G	pET30
pp65_336-448	CGC GGA TCC ACC GTG GAA CTG CGT CAG TA	CGC CTC GAG CGA CGT GCA CGC CGT CGC	pET30
pp65_336-439	CGC GGA TCC ACC GTG GAA CTG CGT CAG TA	CGC CTC GAG TGA TTT GCG TTT GCG GCC	pET30
pp65_336-422	CGC GGA TCC ACC GTG GAA CTG CGT CAG TA	CGC CTC GAG GCC GGTGAC GCG GGG CGT	pET30
murine C3d	CGC GAT ATC ACC CCC GCA GGC TGT GGG GAA C 3'	CGC GGA TCC GGA TCC GCT ACG GCT GGG GAG	pET32
pp65_336-439	CGC GGA TCC ACC GTG GAA CTG CGT CAG TA	CGC CTC GAG TGA TTT GCG TTT GCG GCC	pAS-1

The underlined sequences mean the usage of restrictive enzyme
Hsieh et al. Arthritis Research & Therapy 2011 13:R162 doi:10.1186/ar3481

Table 1