The cross-reactivity of purified anti-pp65336-439 or anti-pp651-167 antibody to dsDNA. (a) ELISA assays for affinity-purified anti-pp65336-439 antibody (pp65336-439), anti-pp651-167 antibody (pp651-167), flow through and SLE patients' sera (SLE) against dsDNA. Graphs depict mean ± s.e.m. values. Unpaired Student t test was performed. Results with a P-value of < 0.05 were considered to be significant. (b) Representations of Crithidia luciliae stain by affinity-purified anti-pp65336-439 antibody (b1, pp65336-439), anti-pp651-167 antibody (b2, pp651-167), flow through (b3) and SLE patients' sera (b4, SLE) against dsDNA. White arrowheads indicate the positive stains. (c) Representations of Immunofluorescent stain for immunoglobulin deposition on glomerular by pp65336-439 (c1) immunized mice, pp651-167 (c2) immunized mice, SA-C3d (c3) immunized mice or PBS (c4) controls. Kidneys were collected at 20 weeks of age. White arrowheads indicate antibody deposition on glomerular. dsDNA: double-stranded DNA.
Hsieh et al. Arthritis Research & Therapy 2011 13:R162 doi:10.1186/ar3481