Figure 5.

Detection of anti-nuclear reactivity in pp65_1-167 or pp65_336-439 specific antibodies from eight weeks post-immunization sera. (a) ELISA assays of affinity purified anti-pp65_336-439 antibody (pp65_336-439), anti-pp65_1-167 antibody (pp65_1-167), SLE patients' sera (SLE) and flow through against purified HCMV virions. Graphs depict mean ± s.e.m. values. Unpaired Student t test was performed. Results with a P-value of < 0.05 were considered to be significant. (b) Immunoblot assays on affinity purified anti-pp65_336-439 antibody (pp65_336-439), anti-pp65_1-167 antibody (pp65_1-167), SLE patients' sera (SLE) and flow through against purified HCMV virions. Molecular mass markers (kD) are shown on the left. MW: molecular weight. (c) ANA stains with HEp-2 substrate slides were performed with HCMV affinity-purified antibody. Patterns of speckle (c₁, c₇), chromatin (c₂, c₈), centriole (c₃, c₉) and MSA II (c₄, c₁₀) were revealed with affinity purified anti-pp65_336-439 antibodies. Nuclear pattern was not found in flow through (c₅, c₁₁) or affinity-purified anti-pp65_1-167 antibody (c₆, c₁₂) stains. White arrowheads indicate the patterns of nuclear response. MSA II: mitotic spindle type II.