Figure 5.

Detection of anti-nuclear reactivity in pp651-167 or pp65336-439 specific antibodies from eight weeks post-immunization sera. (a) ELISA assays of affinity purified anti-pp65336-439 antibody (pp65336-439), anti-pp651-167 antibody (pp651-167), SLE patients' sera (SLE) and flow through against purified HCMV virions. Graphs depict mean ± s.e.m. values. Unpaired Student t test was performed. Results with a P-value of < 0.05 were considered to be significant. (b) Immunoblot assays on affinity purified anti-pp65336-439 antibody (pp65336-439), anti-pp651-167 antibody (pp651-167), SLE patients' sera (SLE) and flow through against purified HCMV virions. Molecular mass markers (kD) are shown on the left. MW: molecular weight. (c) ANA stains with HEp-2 substrate slides were performed with HCMV affinity-purified antibody. Patterns of speckle (c1, c7), chromatin (c2, c8), centriole (c3, c9) and MSA II (c4, c10) were revealed with affinity purified anti-pp65336-439 antibodies. Nuclear pattern was not found in flow through (c5, c11) or affinity-purified anti-pp651-167 antibody (c6, c12) stains. White arrowheads indicate the patterns of nuclear response. MSA II: mitotic spindle type II.

Hsieh et al. Arthritis Research & Therapy 2011 13:R162   doi:10.1186/ar3481
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