Expression of ChemR23 in rheumatoid arthritis and osteoarthritis synovial tissue samples. ChemR23 expression in synovial tissue samples examined by immunohistochemistry that were obtained from five rheumatoid arthritis (RA) patients (A) and three osteoarthritis (OA) patients (B). All sections were counterstained with hematoxylin. Sections of RA synovial tissues were also double-stained with CD68, CD1a, DC-LAMP or vimentin, and ChemR23 and were analyzed by fluorescence microscopy as follows: (C) CD68, (D) ChemR23, (E) merged image of (C) and (D), (F) CD1a, (G) ChemR23, (H) merged image of (F) and (G), (I) DC-LAMP, (J) ChemR23, (K) merged image of (I) and (J), (L) vimentin, (M) ChemR23 and (N) merged image of (L) and (M). Arrows indicate double-positive cells. Original magnification ×100 in (A) and (B) and ×400 in (C) through (N). Whole-mount specimens obtained from RA patients were stained with anti-ChemR23 pAb (green) or phalloidin (blue) and then analyzed by confocal laser-scanning microscopy. (O) x-y view of synovial tissue sectioning. x-z image (P) and y-z image (Q) of synovial tissue sectioning are displayed below and to the right of the x-y view in (O). Scale bars = 80 μm. White straight line boxes indicate identical cell in each direction (O-Q). Dashed line connects the boxes.
Kaneko et al. Arthritis Research & Therapy 2011 13:R158 doi:10.1186/ar3475