Protein expression of hRasGRP4 in the peripheral blood evaluated by immunoblotting. A. Immunoblotting of peripheral blood mononuclear cells from patients with RA and healthy controls. Lysates were prepared from peripheral blood mononuclear cells from patients with rheumatoid arthritis (RA) and healthy controls. Approximately 3 μg of protein from each lysate was subjected to SDS-PAGE. Immunoblotting was performed using peroxidase-conjugated anti-hRasGRP4 antibodies and anti-β-actin antibodies. PBMCs from four healthy individuals and six RA patients were evaluated in panels A. B. Immunoblotting of circulating CD14+ cells isolated from five healthy controls and six RA patients.
Hashimoto et al. Arthritis Research & Therapy 2011 13:R154 doi:10.1186/ar3470