Table 2

Effect of CNP on gene expression in chondrocyte/agarose constructs

100 pM

100 nM

UT

IL-1β

CNP

IL-1β + CNP

IL-1β + CNP + KT5823

CNP

IL-1β + CNP

IL-1β + CNP + KT5823


Culture period (hr)

6

48

6

48

6

48

6

48

6

48

6

48

6

48

6

48

Collagen type I

a

a

ND

ND

b

c

ND

ND

ND

ND

a

d

ND

ND

ND

ND

Collagen type II

b

a

d

d

a

a

a

a

a

a

a

c

d

d

a

a

Col X

a

a

a

a

b

c

b

c

d

d

a

a

a

a

a

a

Sox-9

a

a

ND

ND

a

a

ND

ND

ND

ND

b

c

ND

ND

ND

ND

MMP-13

a

a

b

c

b

b

b

c

d

d

a

a

d

d

a

a


aNo significant change; ↑b, a significant increase (P < 0.05); ↑c, a significant increase (P < 0.01); ↓d, a significant decrease (P < 0.05); ND, not determined. Chondrocyte/agarose constructs were cultured with low (100 pM) or high (100 nM) concentrations of CNP with 0 or 10 ng/ml IL-1β and/or 5 μM KT5823 for 6 and 48 hours (n = 8). Note that statistical comparisons were determined for the following samples: Time = 0 versus 6 or 48 hr (UT); UT versus IL-1β; UT versus CNP; IL-1β versus IL-1β + CNP; IL-1β + CNP versus IL-1β + CNP + KT5823.

Ramachandran et al. Arthritis Research & Therapy 2011 13:R145   doi:10.1186/ar3459

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