Figure 6.

Immunostaining for Osterix, Dlx5, and AP-1 in the OLF. Osterix (a), Dlx5 (b), and AP-1 (c) were strongly positive in hypertrophic chondrocytes (scale bar = 100 μm; n.c., negative control; black arrow, hypertrophic chondrocytes; black arrowheads, proliferating chondrocytes). OLF, ossification of the ligamentum flavum.