Table 2

Summary of differentially expressed genes in the lymph node cells of different groups of Lewis rats tested ex vivo and in vitroa

DEGs, n (%)

Characteristics

Upregulation

Downregulation

Total


Ex vivo

Inc/Nv

322 (100%)

0 (0%)

322

Pk/Nv

31 (53.4%)

27 (46.6%)

58

Rec/Nv

28 (80%)

7 (20%)

35

In vitro

Preclinical arthritis rats

41 (67.2%)

20 (32.8%)

61

Preclinical Bhsp65-tolerized rats

579 (98%)

12 (2%)

591


aDEG: differentially expressed gene. "Ex vivo" group shows DEGs in lymph node cells (LNCs) at incubation (Inc), peak (Pk) or recovery (Rec) phase compared to naïve (Nv) state; "in vitro" group shows DEGs induced by mycobacterial heat shock protein 65 (Bhsp65) compared to baseline control (LNCs cultured in medium alone). In Bhsp65-tolerized group, only 76 (12.86%) of the 579 DEGs were related to immune activity (innate immunity, cell-mediated immunity and humoral immunity). The major groups comprised genes relating to cellular proliferation 127 (21.5%) and metabolism 137 (23.2%).

Yu et al. Arthritis Research & Therapy 2011 13:R143   doi:10.1186/ar3457

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