Figure 4.

Comparative gene expression profiles of antigen-stimulated lymph node cells of mycobacterial heat shock protein 65-tolerized rats versus preclinical arthritis rats. A heat map representation of the DEGs induced by mycobacterial heat shock protein 65 (Bhsp65) in LNCs is shown. (A) LNCs harvested from Lewis (LEW) (RT.1^l) rats on day 7 after heat-killed Mycobacterium tuberculosis H37Ra (Mtb) injection (preclinical AA) were cultured in vitro for 24 hours with or without Bhsp65. (B) LNCs of Bhsp65-tolerized rats were processed in the same manner. The results were compared as indicated. (A) represents part of Figure 3 and is included for easy comparison with (B). Red represents upregulated expression, and green indicates downregulated expression. (C) The number of overlapping DEGs in different groups of rat LNCs tested. AA: adjuvant arthritis; DEG: differentially expressed gene; LNC: lymph node cell; Med: medium.