Table 1

Multiple reaction monitoring transitions for liquid chromatography-tandem mass spectrometry assay of eicosanoids

Compound

MRM, m/z

Collision energy, eV

Declustering potential


PGE1

353 → 317

-30

-80

6-keto PGF1α

369 → 163

-35

-100

PGD2

351 → 271

-25

-40 and -80

PGE2

351 → 271

-25

-40 and -80

351 → 175

-30

-80

PGF2α

353 → 193

-35

-90

11β-PGF2α

353 → 309

-20

-60

PGF2β

353 → 309

-25

-40

PGJ2

333 → 189

-25

-40

333 → 271

-25

-80

15-deoxy-Δ-12,14 PGJ2

315 → 271

-20

-90

13,14-dihydro-15-keto PGD2

351 → 207

-27

-80

13,14-dihydro-15-keto PGE2

351 → 333

-17

-80

13,14-dihydro-15-keto PGF2α

353 → 113

-40

-100

TXB2

369 → 195

-23

-80

369 → 169

-25

-90

LTB4

335 → 195

-20

-100

5(S)-HETE

319 → 115

-20

-40

8(S)-HETE

319 → 155

-20

-80

11(S)-HETE

319 → 167

-20

-80

12(S)-HETE

319 → 179

-20

-80

15(S)-HETE

319 → 175

-20

-80

LTD4

495 → 177

-30

-100

LTE4

438 → 333

-25

-100

LXA4

351 → 235

-20

-80

16,16-dimethyl PGF2α (IS)

381 → 319

-35

-100


eV, electron volts; HETE, hydroxyeicosatetraenoic acid; IS, internal standard; LT, leukotriene; LX, lipoxin; MRM, multiple reaction monitoring; m/z, mass/charge; PG, prostaglandin; TX, thromboxane.

de Grauw et al. Arthritis Research & Therapy 2011 13:R123   doi:10.1186/ar3427

Open Data