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Open Access Research article

A targeted lipidomics approach to the study of eicosanoid release in synovial joints

Janny C de Grauw1*, Chris HA van de Lest2 and Paul René van Weeren1

Author Affiliations

1 Department of Equine Sciences, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 114, 3584 CM, Utrecht, The Netherlands

2 Department of Biochemistry and Cell Biology, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 2, 3584 CM, Utrecht, The Netherlands

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Arthritis Research & Therapy 2011, 13:R123  doi:10.1186/ar3427

Published: 27 July 2011

Additional files

Additional file 1:

Calibration lines - Standard curve equations and correlation coefficients for LC-ESI-MS/MS analysis of eicosanoid standards. Calibration lines for liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) analysis were prepared by diluting stock solutions to final concentrations of 100 pg/μL, 50 pg/μL, 25 pg/μL, 10 pg/μL, 5 pg/μL, 2 pg/μL and 1 pg/μL. The internal standard (IS; 16,16-dimethyl prostaglandin F2α) was prepared in ethanol (2 ng/μL) and added to all composite standards at a final concentration of 100 pg/μL.

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