Cytokine levels in serum of nonarthritic IIJ mice and arthritic IIJ mice at early and late stages of disease. Serum was isolated from AR and NAR littermates, and the levels of six cytokines were measured by cytometric bead array. Only (A) IL-6 and (B) TNF-α were detected. IL-12p70, IL-10, monocyte chemotactic protein 1 and IFNγ were measured but not detected. Early AR mice were < 2.5 months old and had had arthritis for < 5 weeks. Late AR mice were > 4 months old and had had arthritis for > 11 weeks. Dashed horizontal line represents the minimum level of detection. Solid horizontal line represents the average per group. **P < 0.01. Filled circles, squares, and triangles represent data points from NAR. Early AR, and Late AR mice, respectively. Serum IL-17A was measured by standard ELISA but was not detected (data not shown).
Adipue et al. Arthritis Research & Therapy 2011 13:R114 doi:10.1186/ar3399