Figure 3.

Analysis of S100A8/A9 staining in different leukocyte populations by confocal microscopy. Cells were isolated, fixed, permeabilized and stained for S100A8/A9 with the 27E10 antibody. T cells, B cells and mDCs (A, C and E, respectively) had no detectable S100A8/A9 expression while PMNs, pDCs and monocytes (B, D and F, respectively) had membrane-associated, as well as intracellular, S100A8/A9.