Table 1

Sequence of primers for quantitative real time PCR

Primer

Orientation

Sequence

Annealing temp and time

Accession no.

18SrRNA

Qiagen QuantiTect Primer Assay

62°C, 40 sec

[GenBank:X03205]

β-actin

Forward

5'-TCCATCATGAAGTGTGACGTGGAC-3'

62°C, 40 sec

[GenBank:NM_001101]

Reverse

5'-TTGATCTTCATTGTGCTGGGTGCC-3'

BMP-2

Qiagen QuantiTect Primer Assay

60°C, 40 sec

[GenBank::NM_001200]

GAPDH

Forward

5'-TGGACTCCACGACGTACTCAG-3'

62°C, 40 sec

[GenBank:NM_002046]

Reverse

5'-CGGGAAGCTTGTCATCAATGGAA-3'

Gremlin-1

Forward

5'-ATACCTGAAGCGAGACTGGTGCAA-3'

64°C, 40 sec

[GenBank:NM_013372]

Reverse

5'-AACAGAAGCGGTTGATGATGGTGC-3'

IL-6

Forward

5'-GTCAATTCGTTCTGAAGAGGTGAGT-3'

64°C, 40 sec

[GenBank:NM_000600]

Reverse

5'-CCCCAGGAGAAGATTCCAAAGATG-3'

IL-8

Forward

5'-AGACATACTCCAAACCTTTCCACCC-3'

58°C, 30 sec

[GenBank:NM_000584]

Reverse

5'-ATTTCTGTGTTGGCGCAGTGTGGT-3'

LIF-1

Forward

5'-TAAGGAGGCCTCGCAGGATGTC-3'

64°C, 30 sec

[GenBank:NM_002309]

Reverse

5'-TAGTCGTGTACCTTGGCACCTC-3'

Chubinskaya et al. Arthritis Research & Therapy 2011 13:R55   doi:10.1186/ar3300