Additional file 2.

Figure S2. Effect of glucosamine on osteoclast differentiation in vitro. Bone marrow cells from healthy mice were isolated, resuspended at 1 × 106/ml in MEM medium (Lonza, Verviers, Belgium) containing 10% FCS and 50 ng/ml of macrophage colony-stimulating factor and cultured for one day. Osteoclasts were generated after six days of culture with 100 ng/ml RANKL and 50 ng/ml macrophage colony-stimulating factor, in the absence or the presence of increasing concentrations of glucosamine (starting from 5 till 100 μg/ml). Tartrate-resistant acid phosphatase (TRAP) on osteoclasts was determined by TRAP staining kit (Sigma-Diagnostics, Charleston, WV, USA). The number of TRAP-positive cells was counted and the data are expressed as the mean ± SD from three independent experiments.

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Ivanovska and Dimitrova Arthritis Research & Therapy 2011 13:R44   doi:10.1186/ar3283