Table 2

Antibodies used to detect CCR4⁺CCR6⁺ Th and Treg cells in PBMCs and phenotype BMSCs by flow cytometry

Antibody

Isotype

Clone/fluorochrome

Concentration (μg/ml)

Source

CD105 (ENG)^a

Mouse IgG₁

266/FITC

10

Becton Dickinson (Bedford, MA, USA)

CD73 (NT5E)^a

Mouse IgG₁

AD2/FITC

20

Becton Dickinson (Bedford, MA, USA)

CD90 (THY1)^a

Mouse IgG₁

5E10/FITC

1

Becton Dickinson (Bedford, MA, USA)

CD34^a

Mouse IgG₁

581/FITC

50

Southern Biotech (Birmingham, AL, USA)

CD45 (PTPRC)^a

Mouse IgG₁

HI30/FITC

10

Caltag (Burlingame, CA, USA)

CD14^a

Mouse IgG₁

61D3/PE

200

Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA)

HLA-DR^a

Mouse IgG_2a

L243/FITC

200

Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA)

CD4^b

Mouse IgG_2b

OKT4/FITC

12

eBioscience (San Diego, CA, USA)

CD25^b

Mouse IgG_1,κ

BC96/APC

25

eBioscience (San Diego, CA, USA)

FOX-P3^b

Mouse IgG₁

236A/E7/PE

50

eBioscience (San Diego, CA, USA)

CCR4^b

Mouse IgG_1,κ

1G1/PE-Cy7

200

BD Pharmingen™ (Basel, Switzerland)

CCR6 (CD196)^b

Mouse IgG_1,κ

11A9/PE

200

BD Pharmingen™ (Basel, Switzerland)

^aAntibodies used to phenotype bone marrow-derived mensenchymal stem cells (BMSCs) by flow cytometry. ^bAntibodies used to detect CCR4⁺CCR6⁺ CD4⁺ T-helper (Th) cells and forkhead box P3-positive regulatory T (Treg) cells by flow cytometry. PBMC, peripheral blood mononuclear cell.

Wu et al. Arthritis Research & Therapy 2011 13:R29   doi:10.1186/ar3257