Transforming growth factor beta 1 (TGFβ1) enhances TGFβ receptors mRNA turnover. Subconfluent human articular chondrocytes (HAC) were incubated with DMEM + 2% FCS for 24 hours. Thereafter, transforming growth factor beta 1 (TGFβ1) or vehicle were added in the presence of actinomycin D (10 μg/ml). Cells were then harvested at the indicated times for RT-PCR.
Baugé et al. Arthritis Research & Therapy 2011 13:R23 doi:10.1186/ar3247