E-selectin-specific signal can be co-registered with plain X-ray imaging in collagen-induced arthritis. Following onset of arthritis induced with bovine collagen, mice were injected with either anti-E-selectin or anti-DNP antibodies labelled with Dylight 750 nm near infrared fluorophore (5 μg intravenously). (a) Data are mean ± standard error of the mean (SEM) of mean fluorescence intensity (MFI) for arthritic and healthy animals (non-immunised controls), and were analysed by two-way ANOVA versus arthritic anti-E-selectin antibody-injected mice: ***P < 0.001. (b) Representative image of mouse with hind paw arthritis and corresponding image co-registered with X-ray following subtraction of background fluorescence. Clinical scores of imaged paws are shown. (c) The corresponding quantification of MFI at 8 hours for either healthy (non-arthritic) animals or mice with clinically definite arthritis and paw thickness ≥2.2 mm. Background fluorescence levels from uninflamed paws have been subtracted. Data are mean ± SEM, and were analysed by one-way ANOVA versus arthritic anti-E-selectin-injected mice: **P < 0.01, ***P < 0.001. Reproduced with permission from .
Gompels and Paleolog Arthritis Research & Therapy 2011 13:201 doi:10.1186/ar3197