Improved renal lesions in female apoE-/-Fas-/- mice treated with L-4F or L-4F plus pravastatin. (a) Glomeruli of female mice treated with L-4F or L-4F plus pravastatin had smaller glomerular tufts compared to vehicle controls as seen in representative fields of renal cortex from each group (top panel; PAS stain; magnification ×400) and enlarged images from the corresponding field (bottom panel). Bars = 25 μm. In addition, the average number of infiltrated glomerular cells reflected this trend. (b) Quantification of glomerular tuft showed mice treated with L-4F or L-4F plus pravastatin had significantly decreased glomerular tuft area compared to vehicle controls (6,845 ± 1,060 and 6,226 ± 1,007 μm2 vs. 7,645 ± 1,200 μm2, respectively). (c) Immunofluorescence staining showed decreased deposition of IgG and C3 within kidneys of L-4F-treated mice compared to vehicle controls. (d) Starting Week 20 of treatment and through euthanasia, L-4F-treated mice had significantly lower levels of proteinuria compared to vehicle controls. *P ≤ 0.05; **P ≤ 0.01.
Woo et al. Arthritis Research & Therapy 2010 12:R93 doi:10.1186/ar3020