BAY11 inhibits mouse pDC-derived IFN-α production and serum IFN-α elevation in vivo. (A, B) Sorted mouse splenic pDCs were preincubated for one hour with different concentrations of BAY11 (10-9 to 10-6 M) or vehicle. The pDCs were then cultured for a further 24 hours with poly U in complex with lipofectamine. (A) The concentrations of IFN-α in the culture supernatants were measured by ELISA. Data are shown as mean ± SEM of five independent experiments. (B) Viable cells were measured by a trypan-blue exclusion test. Similar results were observed in three independent experiments and the results of a representative experiment are shown. (C) C57BL/6 mice pretreated intraperitonealy with BAY11 (10 mg/kg or 5 mg/kg bodyweight) or vehicle for one hour, followed by intravenous injection of 50 μg/head poly U. Serum IFN-α was measured at several time points (one, three, and six hours). Bars indicate means of five independent experiments (except at six hours: n = 4). Statistical significance was determined using Mann-Whitney test (*P < 0.05 **P < 0.01).
Miyamoto et al. Arthritis Research & Therapy 2010 12:R87 doi:10.1186/ar3014