Figure 3.

BAY11 inhibits IFN-α production from human pDCs. Human pDCs were preincubated for 15 minutes with different concentrations of BAY11 (10^-9, 3 × 10^-9, 10^-8, 3 × 10^-8, 10^-7, 3 × 10^-7) or vehicle. CpG 2216 or loxo were then added to the pDC cultures. (A) After 24 hours, the concentrations of IFN-α and TNF-α in the culture supernatants were measured by ELISA. Data are shown as mean ± SEM of four independent donors. Statistical significance was determined using Mann-Whitney test (*P < 0.05, **P < 0.01). (B) After eight hours of stimulation with CpG 2216, intracellular cytokine (IFN-α and TNF-α) expression and surface BDCA4 expression by pDCs were analyzed by flow cytometry. Percentages of the cytokine-producing pDCs are indicated in each dot-blot profile. Similar results were observed in three independent donors and the results of a representative experiment are shown.