Table 1

Description of the sequences used to quantify gene expression and real-time reaction efficiencies of PCR assays

Gene

Gene ID

Sequences

Product size (bp)

Efficiency


MMP-3

396769

Forward: 5'-ACCCAAGAAGTATCCACACCCT-3'

215

1.98 ± 0.06

Reverse: 5'-TGCTTCAAAGACAGCATCCACT-3'

MMP-13

397346

Forward: 5'-CCAAAGGCTACAACTTGTTTCTTG-3'

77

1.99 ± 0.03

Reverse: 5'-TGGGTCCTTGGAGTGGTCAA-3'

Collagen type II

397323

Forward: 5'-CGCTGAACATCCTCACAAC-3'

249

1.98 ± 0.19

Reverse: 5'-TCCTGTAGATACGCCTAAGC-3'

Fibronectin

397620

Forward: 5'-GACAGATGAGCTTCCCCAAC-3'

752

2.02 ± 0.09

Reverse: 5'-CACTGCCAAAGCCTAAGCAC-3'

GAPDH

396823

Forward: 5'-AATCCCATCACCATCTTCCA-3'

318

2.03 ± 0.01

Reverse: 5'-TGTGGTCATGAGTCCTTCCA-3'


Primers used in quantitative polymerase chain reaction (qPCR) experiments with SYBR green produced amplicons of 77 to 752 base pairs with efficiency values between 1.98 and 2.03. GAPDH, glyceraldehyde 3-phosphate dehydrogenase; MMP-3, matrix metalloproteinase-3; MMP-13, matrix metalloproteinase-13.

Chowdhury et al. Arthritis Research & Therapy 2010 12:R82   doi:10.1186/ar3009

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