Table 1 |
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|
Description of the sequences used to quantify gene expression and real-time reaction efficiencies of PCR assays |
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|
Gene |
Gene ID |
Sequences |
Product size (bp) |
Efficiency |
|
|
||||
|
MMP-3 |
||||
|
Forward: 5'-ACCCAAGAAGTATCCACACCCT-3' |
215 |
1.98 ± 0.06 |
||
|
Reverse: 5'-TGCTTCAAAGACAGCATCCACT-3' |
||||
|
MMP-13 |
||||
|
Forward: 5'-CCAAAGGCTACAACTTGTTTCTTG-3' |
77 |
1.99 ± 0.03 |
||
|
Reverse: 5'-TGGGTCCTTGGAGTGGTCAA-3' |
||||
|
Collagen type II |
||||
|
Forward: 5'-CGCTGAACATCCTCACAAC-3' |
249 |
1.98 ± 0.19 |
||
|
Reverse: 5'-TCCTGTAGATACGCCTAAGC-3' |
||||
|
Fibronectin |
||||
|
Forward: 5'-GACAGATGAGCTTCCCCAAC-3' |
752 |
2.02 ± 0.09 |
||
|
Reverse: 5'-CACTGCCAAAGCCTAAGCAC-3' |
||||
|
GAPDH |
||||
|
Forward: 5'-AATCCCATCACCATCTTCCA-3' |
318 |
2.03 ± 0.01 |
||
|
Reverse: 5'-TGTGGTCATGAGTCCTTCCA-3' |
||||
|
|
||||
|
Primers used in quantitative polymerase chain reaction (qPCR) experiments with SYBR green produced amplicons of 77 to 752 base pairs with efficiency values between 1.98 and 2.03. GAPDH, glyceraldehyde 3-phosphate dehydrogenase; MMP-3, matrix metalloproteinase-3; MMP-13, matrix metalloproteinase-13. |
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|
Chowdhury et al. Arthritis Research & Therapy 2010 12:R82 doi:10.1186/ar3009 |
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