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Open Access Highly Accessed Research article

Altered microRNA expression profile with miR-146a upregulation in CD4+ T cells from patients with rheumatoid arthritis

Jingyi Li12, Ying Wan1, Qiuye Guo1, Liyun Zou1, Jinyu Zhang1, Yongfei Fang2, Jingbo Zhang1, Jinjun Zhang1, Xiaolan Fu1, Hongli Liu1, Liwei Lu3 and Yuzhang Wu1*

Author Affiliations

1 Institute of Immunology, PLA, Third Military Medical University, 30# Gaotanyan Street, District Shipingba, Chongqing 400038, PR China

2 Department of Rheumatology, Southwest Hospital, Third Military Medical University, Chongqing, 30# Gaotanyan Street, District Shipingba, Chongqing 400038, PR China

3 Department of Pathology and Center of Infection and Immunology, The University of Hong Kong, Pokfulam Road, Hong Kong, PR China

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Arthritis Research & Therapy 2010, 12:R81  doi:10.1186/ar3006

Published: 11 May 2010

Additional files

Additional file 1:

Levels of Th1/Th2 cytokines in serum and SF of normal controls and RA patients. Levels of Th1/Th2 cytokines in serum and synovial fluid (SF) of normal controls and rheumatoid arthritis (RA) patients. Levels of IL-2, IFN-α, IL-10, IL-4, IL-6 and TNF-α were up-regulated in SF of RA patients. Ctl -PB (n = 5): serum of healthy controls; RA-PB (n = 15): serum of RA patients; RA-SF (n = 36): synovial fluid of RA patient; **P < 0.01, *P < 0.05.

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Additional file 2:

Correlations between miRNAs and levels of cytokines in peripheral blood and SF of RA patients. Expression of miR-498, miR-363 and miR-150 did not show any correlation with levels of cytokine (TNF-α, IL-2, IFN-α, IL-4, IL-6 and IL-10) production. Correlations between miRNAs (miR-498, miR-363, miR-150) and cytokines (TNF-α, IL-2, IFN-α, IL-4, IL-6 and IL-10) were analyzed by statistical evaluation and no significant results were found.

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Additional file 3:

Expression of various cytokines in freshly prepared CD4+ T cells transfected with FUGW-miR-146a. Flow cytometric analysis did not detect any significant alteration in the expression levels of IFN-γ, TNF-α, IL-2 and IL-17A in normal CD4+ T cells transfected with miR-146a. Expression levels of IFN-γ, TNF-α, IL-2 and IL-17A were assayed by intracellular cytokine staining, but no significant change was detected between FF3-and miR-146a-CD4+ T cells.

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