Research article
Altered microRNA expression profile with miR-146a upregulation in CD4+ T cells from patients with rheumatoid arthritis
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* Corresponding author: Yuzhang Wu wuyuzhang@yahoo.com
- † Equal contributors
1 Institute of Immunology, PLA, Third Military Medical University, 30# Gaotanyan Street, District Shipingba, Chongqing 400038, PR China
2 Department of Rheumatology, Southwest Hospital, Third Military Medical University, Chongqing, 30# Gaotanyan Street, District Shipingba, Chongqing 400038, PR China
3 Department of Pathology and Center of Infection and Immunology, The University of Hong Kong, Pokfulam Road, Hong Kong, PR China
Arthritis Research & Therapy 2010, 12:R81 doi:10.1186/ar3006
Published: 11 May 2010Additional files
Additional file 1:
Levels of Th1/Th2 cytokines in serum and SF of normal controls and RA patients. Levels of Th1/Th2 cytokines in serum and synovial fluid (SF) of normal controls and rheumatoid arthritis (RA) patients. Levels of IL-2, IFN-α, IL-10, IL-4, IL-6 and TNF-α were up-regulated in SF of RA patients. Ctl -PB (n = 5): serum of healthy controls; RA-PB (n = 15): serum of RA patients; RA-SF (n = 36): synovial fluid of RA patient; **P < 0.01, *P < 0.05.
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Additional file 2:
Correlations between miRNAs and levels of cytokines in peripheral blood and SF of RA patients. Expression of miR-498, miR-363 and miR-150 did not show any correlation with levels of cytokine (TNF-α, IL-2, IFN-α, IL-4, IL-6 and IL-10) production. Correlations between miRNAs (miR-498, miR-363, miR-150) and cytokines (TNF-α, IL-2, IFN-α, IL-4, IL-6 and IL-10) were analyzed by statistical evaluation and no significant results were found.
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Additional file 3:
Expression of various cytokines in freshly prepared CD4+ T cells transfected with FUGW-miR-146a. Flow cytometric analysis did not detect any significant alteration in the expression levels of IFN-γ, TNF-α, IL-2 and IL-17A in normal CD4+ T cells transfected with miR-146a. Expression levels of IFN-γ, TNF-α, IL-2 and IL-17A were assayed by intracellular cytokine staining, but no significant change was detected between FF3-and miR-146a-CD4+ T cells.
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