Table 1

Thermal cycling conditions for real time PCR

Gene

Sequences

Temp (°C)

Amplicon size (bp)


Aggrecan

Fw TCG AGG ACA GCG AGG CC

Rv TCG AGG GTG TAG CGT GTA GAG A

61

85

Collagen type 2

Fw ATG ACA ATC TGG CTG CCA

Rv CTT CAG GGC AGT GTA CGT

58

200

IGF-1

Fw GTA TTG CGC ACC CCT CAA

Rv TTG TTT CCT GCA CTC CCT CT

57

126

TGFβ

Fw TGC GGC AGC TGT ACA TTG A

Rv TGG TTG TAC AGG GCC AGG A

59

186

MMP-13

Fw TGG TGG TGA TGA AGA TGA TTT G

Rv TCT AAG CCG AAG AAA GAC TGC

57

125

TIMP-2

Fw CTT CTT TCC TCC AAC GTC

Rv AAA GCG GTC AGT GAG AAG GA

57

183

COX-2

Fw GCT GGA ACA TGG AAT TAC CCA

Rv CTT TCT GTA CTG CGG GTG GAA

58

98

iNOS

Fw ACA AGC CTA CCC CTC CAG AT

Rv TCC CGT CAG TTG GTA GGT TC

59

157

IL-1

Fw GGA CAA GCT GAG GAA GAT GC

Rv TCG TTA TCC CAT GTG TCG AA

57

120

RP 29

Fw AAG ATG GGT CAC CAG CAG GTC TAC TG

Rv AGA CGC GGC AAG AGC GAG AA

59

153


bp, base pairs; COX-2, cyclooxygenase-2; IGF-1, insulin growth factor-1; MMP, matrix metalloproteinase; iNOS, inducible nitric oxide synthase; Temp, temperature; TGF, transforming growth factor; TIMP, tissue inhibitor of metalloproteinase; IL-1, interleukin-1.

Pallu et al. Arthritis Research & Therapy 2010 12:R112   doi:10.1186/ar3048

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