Additional file 1.
Supplementary figure S1. Apremilast has no effect on cell viability in human cells. (a) Human monocytes and (b) rheumatoid arthritis (RA) synovial membrane cells were cultured with increasing concentrations of apremilast or rolipram as in Figure 1. After supernatants were collected for cytokine analysis, 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was added at a final concentration of 0.5 ng/ml for six hours. A 100 μl sample of 10% SDS in 0.01 M HCl was then added overnight before the plate was read on a spectrophotometer at 620 nm. None of the culture conditions assayed altered cell viability relative to cells alone.
Format: PDF Size: 25KB Download file
This file can be viewed with: Adobe Acrobat Reader
McCann et al. Arthritis Research & Therapy 2010 12:R107 doi:10.1186/ar3041