Open Access Research article

Mechanical signals control SOX-9, VEGF, and c-Myc expression and cell proliferation during inflammation via integrin-linked kinase, B-Raf, and ERK1/2-dependent signaling in articular chondrocytes

Priyangi M Perera1, Ewa Wypasek1, Shashi Madhavan1, Birgit Rath-Deschner2, Jie Liu1, Jin Nam1, Bjoern Rath3, Yan Huang1, James Deschner4, Nicholas Piesco5, Chuanyue Wu6 and Sudha Agarwal1*

Author affiliations

1 Biomechanics and Tissue Engineering Laboratory, The Ohio State University, Postle Hall, 305 W 12th Avenue, Columbus, OH 43210, USA

2 Department of Orthodontics, University of Bonn, Welschnonnenstrasse 17, 53111 Bonn, Germany

3 Department of Orthopedics, University of Regensburg, Kaiser-Karl V-Allee 3, 93077 Bad Abbach, Germany

4 Department of Periodontics, University of Bonn, Welschnonnenstrasse 17, 53111 Bonn, Germany

5 Department of Oral Biology, School of Dental Medicine, Salk Hall, 3501 Terrace Street, University of Pittsburgh, Pittsburgh, PA 15261, USA

6 Department of Pathology, University of Pittsburgh, School of Medicine, S-417 BST, 200 Lothrop Street, Pittsburgh, PA 15261, USA

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Citation and License

Arthritis Research & Therapy 2010, 12:R106  doi:10.1186/ar3039

Published: 28 May 2010

Abstract

Introduction

The importance of mechanical signals in normal and inflamed cartilage is well established. Chondrocytes respond to changes in the levels of proinflammatory cytokines and mechanical signals during inflammation. Cytokines like interleukin (IL)-1β suppress homeostatic mechanisms and inhibit cartilage repair and cell proliferation. However, matrix synthesis and chondrocyte (AC) proliferation are upregulated by the physiological levels of mechanical forces. In this study, we investigated intracellular mechanisms underlying reparative actions of mechanical signals during inflammation.

Methods

ACs isolated from articular cartilage were exposed to low/physiologic levels of dynamic strain in the presence of IL-1β. The cell extracts were probed for differential activation/inhibition of the extracellular signal-regulated kinase 1/2 (ERK1/2) signaling cascade. The regulation of gene transcription was examined by real-time polymerase chain reaction.

Results

Mechanoactivation, but not IL-1β treatment, of ACs initiated integrin-linked kinase activation. Mechanical signals induced activation and subsequent C-Raf-mediated activation of MAP kinases (MEK1/2). However, IL-1β activated B-Raf kinase activity. Dynamic strain did not induce B-Raf activation but instead inhibited IL-1β-induced B-Raf activation. Both mechanical signals and IL-1β induced ERK1/2 phosphorylation but discrete gene expression. ERK1/2 activation by mechanical forces induced SRY-related protein-9 (SOX-9), vascular endothelial cell growth factor (VEGF), and c-Myc mRNA expression and AC proliferation. However, IL-1β did not induce SOX-9, VEGF, and c-Myc gene expression and inhibited AC cell proliferation. More importantly, SOX-9, VEGF, and Myc gene transcription and AC proliferation induced by mechanical signals were sustained in the presence of IL-1β.

Conclusions

The findings suggest that mechanical signals may sustain their effects in proinflammatory environments by regulating key molecules in the MAP kinase signaling cascade. Furthermore, the findings point to the potential of mechanosignaling in cartilage repair during inflammation.