Table 3

ERα-mediated suppression of arthritis is associated with decreased cytokine and chemokine levels

IL-1β

(pg/mg protein)

IL-6

(pg/mg protein)

IL-12p40

(pg/mg protein)

KC

(pg/mg protein)

RANTES

(pg/mg protein)


Vehicle:

91 ± 22

6 ± 3

5 ± 2

17 ± 5

3.2 ± 1.2

EE 0.025 mg/kg po:

13 ± 38*

12 ± 11

1.9 ± 0.5*

5 ± 2

0.9 ± 0.2*

EE 0.25 mg/kg po:

23 ± 18*

1.0 ± 0.4*

1.5 ± 0.1*

5 ± 4

0.8 ± 0.4*

EE 2.5 mg/kg po

4 ± 2*

0.3 ± 0.3*

0.1 ± 0.1*

2 ± 0.3

0.2 ± 0.1*

Vehicle:

110.4

10.7

3.7

19.1

3.3

Prednisolone 3 mg/kg po

0.6

1.3

0.1

0.6

0

ERA-63 0.75 mg/kg po

71.4

21.3

2.2

20.5

1.4

ERA-63 1.5 mg/kg po

5.9

0.7

0.8

1.3

0.2

ERA-63 3 mg/kg po

2.9

2.0

0.6

2.3

0.2


Biopsies of four mice (n = 4 per cage) were pooled and processed for cytokine and chemokine detection, thereby yielding three samples per treatment group for Luminex measurement. Results are presented as mean picograms per mg total protein obtained from three measurements per treatment group in the first experiment comparing vehicle with ethinyl estradiol (EE; 0.025, 0.25 and 2.5 mg/kg). The standard error of the mean are given. Statistical analysis was performed with the two-tailed Mann-Whitney test. Statistically significant differences (P ≤ 0.05) when compared with the vehicle treatment group is indicated with an asterisk (*). Next, results are based on one measurement on pooled synovial lysates obtained from 12 animals in total (n = 12 mice; 1 biopsy per mouse) in the second experiment comparing vehicle with ERA-63 (0.75, 1.5 and 3 mg/kg). Levels are expressed as picograms per mg total protein. ER, estrogen receptor; po, orally. Chemokine (C-C motif) ligand 5 also known as CCL5 or RANTES and the murine IL-8 homoloque KC.

Dulos et al. Arthritis Research & Therapy 2010 12:R101   doi:10.1186/ar3032

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