ERA-63 inhibits the tetanus-specific delayed type hypersensitivity response in WT and ERβKO but not in the ERαKO mice. Mice were ovariectomized at day -14. Mice (n = 8 per group) were orally treated daily with ERA-63 at 6 mg/kg or vehicle gelatin-mannitol from day -1 to day 9. At day 0, animals were immunized with tetanus toxoid (TT) in dimethyl dioctadecyl ammonium bromide. At day 7, animals were challenged with TT in Al(OH)3. The left control footpad received vehicle only. (A) At autopsy (forty eight hours later) the uterus was removed and weighed. (B) Twenty-four hours later the left and right hind footpad thickness was measured and the delta mm of antigen-specific footpad swelling was calculated according to the following formula: [(swelling left (mm) minus swelling right (mm)]. Data are representative for three independent experiments. Statistical analysis was performed with the analysis of variance test. ** P ≤ 0.01, *** P ≤ 0.001. ER, estrogen receptor; WT, wild type.
Dulos et al. Arthritis Research & Therapy 2010 12:R101 doi:10.1186/ar3032