Table 1

Unstimulated fibroblast-like synoviocytes of patients with psoriatic arthritis or rheumatoid arthritis express higher levels of nerve growth factor and TrkA

FACS analyses, percentage of cells


Cells

NGF, pg/mL

NGF+

TrkA+

p75+


Unstimulated FLSs (meniscal injury n = 4)

30 ± 5

4.5 ± 0.3

1.33 ± 0.4

0.5 ± 0.05

FLSs + IL-1β (meniscal injury)

110 ± 28a

12.4 ± 2a

16.5 ± 5a

0.4 ± 0.2

FLSs + TNF-a (meniscal injury)

129 ± 23a

6.3 ± 4a

11.5 ± 5a

0.71 ± 0.4

FLSs from patients with PsA (n = 3)

105 ± 15a

17 ± 4a

10 ± 3a

1.0 ± 0.4

FLSs from patients with RA (n = 4)

65 ± 4b

8 ± 0.4b

5.5 ± 0.4b

0.5 ± 0.4

FLSs from patients with OA (n = 4)

34 ± 2

3.9 ± 0.2

0.9 ± 0.2

0.7 ± 0.2


Third-passage fibroblast-like synoviocytes (FLSs) (5,000 cells in 200 μL of Dulbecco's modified Eagle's medium [DMEM] complete medium) in 96-well plates were cultured for 6 days. Unstimulated cells were cultured in DMEM only, and stimulated cells were cultured in DMEM with cytokines tumor necrosis factor-alpha (TNF-α) (1 ng/mL) and interleukin-1-beta (IL-1β) (1 ng/mL). Data are expressed as mean ± standard deviation. Nerve growth factor (NGF), TrkA, and p75 data of unstimulated FLSs were compared with NGF, TrkA, and p75 data of FLSs of other groups mentioned in the table. a/bSignificantly different from unstimulated cells (aP < 0.001, bP < 0.05 Student t test). FACS, fluorescence-activated cell sorting; OA, osteoarthritis; PsA, psoriatic arthritis; RA, rheumatoid arthritis.

Raychaudhuri and Raychaudhuri Arthritis Research & Therapy 2010 12:404   doi:10.1186/ar3030