Examination of lung tissues. (1) The lung tissue staining for intratracheally injected fluorescent siRNA. Intense fluorescence was observed within epithelial cells of bronchi and bronchioles, and weak fluorescence was detected in the parenchyma. (2) Representative histological features of HE and Trichrome stain of mouse lung samples with different treatments intratracheally in low (4 ×) and high magnifications (40 ×). Four mice were used for each group. A. Injection with saline (negative control) only; B. Injection with bleomycin only on Day 0; C. Injection with bleomycin on Day 0 and SPARC siRNA on Days 2, 5, and 12; D. Injection with bleomycin on Day 0 and Ctgf siRNA on Days 2, 5, and 12. (3) Collagen contents in lung samples with different treatments. The collagen content in the lung sample from saline treated mice was normalized to 1. Four mice were used for each group. Treatments: Sa, saline; B, bleomycin; B + C, bleomycin and Ctgf siRNAs; B + S, bleomycin and SPARC siRNA. *, P < 0.05.
Wang et al. Arthritis Research & Therapy 2010 12:R60 doi:10.1186/ar2973