Examination of skin tissues. (1) Representative histological analysis of HE and Trichrome stain of mouse skin with different treatments for four weeks in low (4 ×) and high magnifications (20 ×). Four mice were used for each group. A. Injection with saline (negative control) only; B. Injection with bleomycin only; C. Injection with bleomycin and treatment with SPARC siRNA; D. Injection with bleomycin and treatment with Ctgf siRNA. (2) Collagen contents in skin samples with different treatments. The collagen content in the skin sample from saline treated mice was normalized to 1. Treatments: Sa, saline; B, bleomycin; B + C, bleomycin and Ctgf siRNAs; B + S, bleomycin and SPARC siRNA. P < 0.05.
Wang et al. Arthritis Research & Therapy 2010 12:R60 doi:10.1186/ar2973