Email updates

Keep up to date with the latest news and content from Arthritis Research & Therapy and BioMed Central.

Editorial

Vasculogenesis in rheumatoid arthritis

Zoltán Szekanecz1* and Alisa E Koch23

Author Affiliations

1 Department of Rheumatology, Institute of Medicine, University of Debrecen Medical and Health Sciences Center, 98 Nagyerdei street, Debrecen, H-4032, Hungary

2 Veterans' Administration, Ann Arbor Healthcare System, 109 Zina Pitcher Place, Ann Arbor, MI 48109-2200, USA

3 University of Michigan Health System, Department of Internal Medicine, Division of Rheumatology, 109 Zina Pitcher Place, Ann Arbor, MI 48109-2200, USA

For all author emails, please log on.

Arthritis Research & Therapy 2010, 12:110  doi:10.1186/ar2943


See related research by Jodon de Villeroché et al., http://arthritis-research.com/content/12/1/R27

The electronic version of this article is the complete one and can be found online at: http://arthritis-research.com/content/12/2/110


Published:18 March 2010

© 2010 BioMed Central Ltd

Abstract

Decreased number and impaired functions of endothelial progenitor cells (EPCs) leading to impaired vasculogenesis have been associated with rheumatoid arthritis (RA). Defective vasculogenesis has also been implicated in premature atherosclerosis in RA. Recently, early-outgrowth monocytic and late-outgrowth hemangioblastic EPC subsets have been characterized. Hemangioblastic EPCs may exert increased numbers in active RA and may play a role in vascular repair underlying RA.

Editorial

Endothelial progenitor cells (EPCs) are hematopoietic stem cells expressing CD34, CD133, type 2 vascular endothelial growth factor (VEGF) receptor (VEGFR-2 or Flk-1), and the CXCR4 chemokine receptor [1-4]. During vasculogenesis, EPCs are mobilized from the bone marrow and they differentiate into mature endothelial cells [3]. Under normal conditions, vasculogenesis is involved in both prenatal and postnatal tissue development, vascular repair, and atherosclerosis [2,3].

In rheumatoid arthritis (RA), several groups have described defective vasculogenesis related to impaired EPC numbers and functions in RA [4-6]. Impaired vasculogenesis has been associated with increased cardiovascular morbidity and mortality in RA [7,8]. Effective antirheumatic therapy, such as corticosteroids and tumor necrosis factor-alpha (TNF-α) blockers, may stimulate the outgrowth and function of EPCs and thus may restore defective vasculogenesis in arthritis [5]. In addition, as the induction of vasculogenesis may be beneficial for patients with cardiovascular disease [8], the stimulation of EPCs and vasculogenesis may also suppress premature atherosclerosis underlying RA [7].

In the previous issue of Arthritis Research & Therapy, Jodon de Villeroché and colleagues [1] assessed late-outgrowth EPCs in RA and found increased colony-formation capacity of these cells in RA. Furthermore, higher or lower EPC numbers correlated with active disease and disease in remission, respectively. These results seem to be somewhat controversial as a number of other investigators reported defective EPC function in RA and lower EPC numbers in active RA [5,6]. There has been only one report by the same group, Allanore and colleagues [9], suggesting that circulating EPC numbers may be higher in RA. Nevertheless, Jodon de Villeroché and colleagues [1] conducted an approach that was significantly different from that of others. Instead of analyzing all EPCs, they differentiated two EPC sub-populations, namely EPCs of monocytic versus hemangioblastic origin. These two EPC subsets have recently been described and characterized as early-outgrowth and late-outgrowth EPCs, respectively [1,10]. There is no clear consensus on the accurate definition of EPCs after all [10]. In their study, Jodon de Villeroché and colleagues [1] characterized late-outgrowth EPCs of hemangioblastic origin as Lin-/7-aminoactinomycin D (7AAD)-/CD34+/CD133+/VEGFR-2+ cells and the number of these cells was indeed higher in RA patients compared with controls. In addition, the colony-forming capacity of these late-outgrowth EPCs was significantly higher in RA.

Jodon de Villeroché and colleagues [1] claim that, in all previous studies, EPCs also consisted of the early-outgrowth monocyte-derived cells characterized by only three surface markers (CD34/CD133/VEGF-R2) [5,6]. According to Jodon de Villeroché and colleagues [1], the use of Lin and 7AAD markers may enable investigators to select only late-outgrowth EPCs.

Thus, while there may be a general impairment of EPC function and vasculogenesis in RA and low EPC numbers may be associated with RA activity and increased cardiovascular risk, late-outgrowth EPCs of solely hemangioblastic origin may be involved in vascular repair. As this EPC subset may be preferentially involved in the active stage of the disease, it is likely that hemangioblastic EPC-dependent vasculogenesis is more prominent in active RA associated with high-grade systemic inflammation and accelerated atherosclerosis. Regarding potential relevance for therapy, corticosteroids and anti-TNF agents may, in general, stimulate EPC number and function [5,11] but the possible effects of these agents on the function of late-outgrowth EPCs need further characterization.

Abbreviations

7AAD: 7-aminoactinomycin D; EPC: endothelial progenitor cell; RA: rheumatoid arthritis; TNF: tumor necrosis factor; VEGF: vascular endothelial growth factor; VEGFR-2: type 2 vascular endothelial growth factor receptor.

Competing interests

The authors declare that they have no competing interests.

References

  1. Jodon de Villeroché V, Avouac J, Ponceau A, Ruiz B, Kahan A, Boileau C, Uzan G, Allanore Y: Enhanced late-outgrowth circulating endothelial progenitor cell levels in rheumatoid arthritis and correlation with disease activity.

    Arthritis Res Ther 2010, 12:R27. PubMed Abstract | BioMed Central Full Text OpenURL

  2. Paleolog E: It's all in the blood: circulating endothelial progenitor cells link synovial vascularity with cardiovascular mortality in rheumatoid arthritis?

    Arthritis Res Ther 2005, 7:270-272. PubMed Abstract | BioMed Central Full Text | PubMed Central Full Text OpenURL

  3. Peichev M, Naiyer AJ, Pereira D, Zhu Z, Lane WJ, Williams M, Oz MC, Hicklin DJ, Witte L, Moore MA, Rafii S: Expression of VEGFR-2 and AC133 by circulating human CD34(+) cells identifies a population of functional endothelial precursors.

    Blood 2000, 95:952-958. PubMed Abstract | Publisher Full Text OpenURL

  4. Pakozdi A, Besenyei T, Paragh G, Koch AE, Szekanecz Z: Endothelial progenitor cells in arthritis-associated vasculogenesis and atherosclerosis.

    Joint Bone Spine 2009, 76:581-583. PubMed Abstract | Publisher Full Text OpenURL

  5. Grisar J, Aletaha D, Steiner CW, Kapral T, Steiner S, Saemann M, Schwarzinger I, Buranyi B, Steiner G, Smolen JS: Endothelial progenitor cells in active rheumatoid arthritis: effects of tumour necrosis factor and glucocorticoid therapy.

    Ann Rheum Dis 2007, 66:1284-1288. PubMed Abstract | Publisher Full Text OpenURL

  6. Herbrig K, Haensel S, Oelschlaegel U, Pistrosch F, Foerster S, Passauer J: Endothelial dysfunction in patients with rheumatoid arthritis is associated with a reduced number and impaired function of endothelial progenitor cells.

    Ann Rheum Dis 2006, 65:157-163. PubMed Abstract | Publisher Full Text | PubMed Central Full Text OpenURL

  7. Szekanecz Z, Koch AE: Vascular involvement in rheumatic diseases: 'vascular rheumatology'.

    Arthritis Res Ther 2008, 10:224. PubMed Abstract | BioMed Central Full Text | PubMed Central Full Text OpenURL

  8. Freedman SB, Isner JM: Therapeutic angiogenesis for ischemic cardiovascular disease.

    J Mol Cell Cardiol 2001, 33:379-393. PubMed Abstract | Publisher Full Text OpenURL

  9. Allanore Y, Batteux F, Avouac J, Assous N, Weill B, Kahan A: Levels of circulating endothelial progenitor cells in systemic sclerosis.

    Clin Exp Rheumatol 2007, 25:60-66. PubMed Abstract | Publisher Full Text OpenURL

  10. Ingram DA, Caplice NM, Yoder MC: Unresolved questions, changing definitions, and novel paradigms for defining endothelial progenitor cells.

    Blood 2005, 106:1525-1531. PubMed Abstract | Publisher Full Text OpenURL

  11. Ablin JN, Boguslavski V, Aloush V, Elkayam O, Paran D, Caspi D, George J: Effect of anti-TNFalpha treatment on circulating endothelial progenitor cells (EPCs) in rheumatoid arthritis.

    Life Sci 2006, 79:2364-2369. PubMed Abstract | Publisher Full Text OpenURL