Additional file 2.

GW2580 does not modulate T-cell function in vivo. Splenocytes were harvested from DBA/1 mice with CIA and treated with GW2580, imatinib, or vehicle, and stimulated with 20 μg/ml heat-denatured, whole CII. [3H]thymidine incorporation was used to measure proliferation of CII-specific T cells. IFNγ, TNFα and IL-10 were measured in culture supernatants by ELISA. Values are the mean ± SEM. *P < 0.05 compared with stimulated cells from vehicle-treated CIA mice. Results are representative of 2 independent experiments.

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Paniagua et al. Arthritis Research & Therapy 2010 12:R32   doi:10.1186/ar2940