Figure 2.

Mesenchymal stem cells (MSCs) inhibit the anti-CD3-induced proliferation of CD4+ T cells in vitro. (a) CD4+ T cells (5 × 104 cells) and accessory cells (5 × 104 cells) were incubated with 3 μg/ml anti-CD3 antibody and the indicated numbers of mitomycin c-treated wild-type or interferon-gamma receptor knockout (IFN-γR KO) MSCs for 72 hours and pulsed for the last 16 hours with 1 μCi of [3H]TdR. The percentage inhibition (100 × [(radioactivity in cultures without MSCs -- radioactivity in cultures with MSCs)/radioactivity in cultures without MSCs]) by increasing numbers of MSCs is shown. Each result represents the mean of four cultures ± standard error of the mean (SEM). Results are representative of two independent experiments. * P < 0.05 for comparison with wild-type MSCs (Mann-Whitney U test). (b) Carboxyfluorescein succinimidyl ester (CFSE)-labeled CD4+ T cells (5 × 104 cells) and accessory cells (5 × 104 cells) were incubated with 3 μg/ml anti-CD3 antibody and the indicated numbers of mitomycin c-treated wild-type or IFN-γR KO MSCs for 72 hours. The proliferation of CD4+ T cells was analyzed by detection of CFSE dilution by flow cytometry. The percentage inhibition (100 × [(percentage of proliferating CD4+ cells not treated with MSCs -- percentage of proliferating CD4+ cells treated with MSCs)/percentage of proliferating CD4+ cells not treated with MSCs]) by increasing numbers of MSCs is shown. Each result represents the mean of three cultures ± SEM. Results are representative of two independent experiments. * P < 0.05 for comparison with wild-type MSCs (Mann-Whitney U test).

Schurgers et al. Arthritis Research & Therapy 2010 12:R31   doi:10.1186/ar2939
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