Table 5

NP vs AC and AF microarray comparison

Gene description

Gene symbol

AC mean

AF mean

AC&AF mean

NP mean

Fold change (NP/AC&AF)


keratin 8

KRT8

0.09

0.39

0.28

714.79

2544.79

keratin 18

KRT18

0.01

0.04

0.05

94.10

1860.77

ras homolog gene family, member B

RHOB

0.24

0.04

0.40

383.01

968.68

hypothetical protein BC012029

LOC152573

0.05

0.18

0.26

230.02

897.02

cadherin 2, type 1, N-cadherin

CDH2

0.09

0.22

0.21

180.47

840.69

Kruppel-like factor 6

KLF6

0.13

0.03

0.12

55.84

456.37

plakophilin 2

PKP2

0.13

0.48

0.37

77.71

212.23

related RAS viral (r-ras) oncogene homolog

RRAS

0.74

0.39

0.61

93.40

152.49

synaptosomal-associated protein, 25 kDa

SNAP25

0.55

0.81

0.69

94.22

136.76

sclerostin domain containing 1

SOSTDC1

0.28

0.53

0.68

79.27

116.29

optineurin

OPTN

0.22

0.36

0.80

88.35

109.86

tropomyosin 2 (beta)

TPM2

18.99

94.17

78.02

0.74

-104.73

integrin, alpha 9

ITGA9

38.74

62.19

66.25

0.50

-131.50

pleiotrophin (heparin binding growth factor 8)

PTN

208.08

54.11

167.82

1.10

-152.58

Tissue factor pathway inhibitor 2

TFPI2

1080.55

5089.17

4138.76

26.91

-153.83

guanine nucleotide binding protein (G protein), gamma 11

GNG11

0.57

331.31

298.19

1.65

-181.06

podocalyxin-like

PODXL

0.50

297.24

208.74

1.14

-182.62

proteoglycan 4

PRG4

917.06

27.09

722.28

3.25

-222.06

a disintegrin-like and metallopeptidase with thrombospondin type 1 motif, 4

ADAMTS4

115.72

2187.60

1561.34

5.25

-297.16

phenylalanine hydroxylase

PAH

195.83

0.24

98.50

0.32

-307.92

interferon, gamma-inducible protein 16

IFI16

0.28

61.31

67.55

0.20

-337.80

retinoic acid receptor responder 1

RARRES1

974.75

156.02

671.46

1.98

-338.73

regulator of G-protein signalling 5

RGS5

5.68

436.91

264.83

0.67

-393.62

integrin-binding sialoprotein

IBSP

300.90

0.06

159.69

0.37

-433.73

complement factor H

CFH

1.72

60.74

86.66

0.12

-729.93

fibulin 1

FBLN1

199.29

0.22

108.88

0.13

-826.03

Serpin peptidase inhibitor, clade F, member 1

SERPINF1

17.15

512.43

281.58

0.34

-830.27

Keratocan

KERA

0.05

346.47

341.38

0.38

-893.66

collagen, type X, alpha 1

COL10A1

1192.84

7.19

741.87

0.79

-944.44

Endomucin

EMCN

0.45

281.94

189.76

0.16

-1166.81

secreted frizzled-related protein 2

SFRP2

9.98

153.89

106.73

0.08

-1391.84

peptidase inhibitor 15

PI15

478.32

83.74

308.94

0.10

-3231.26

myosin, heavy chain 11, smooth muscle

MYH11

1.15

462.12

263.70

0.03

-7708.90

chemokine (C-X-C motif) ligand 1

CXCL1

3.14

549.84

492.06

0.02

-28150.12


NP specific marker genes. Genes identified by NP vs AC and AF microarray comparison. Mean fluorescence intensity is shown for each cell type (AC, AF and NP) along with the mean combined intensity for AC and AF cells and the calculated fold change values (positive and negative) between NP and AC and AF cells that were greater than 100. Highlighted in bold are genes that were taken forward for qRT-PCR and italicised are genes that were identified in previous studies [23,24]. AC, articular cartilage; AF, annulus fibrosus; NP, nucleus pulposus; qRT-PCR, quantitative real time polymerase chain reaction.

Minogue et al. Arthritis Research & Therapy 2010 12:R22   doi:10.1186/ar2929

Open Data