Table 1

Ovine-specific real time PCR primer pair sequences, annealing temperatures and product size

Target gene

Sequence

Anneal temp (°C)

Product size (bp)

Accession number or reference if published

Acan

F - TCA CCA TCC CCT GCT ACT TCA TC

R - TCT CCT TGG AAA TGC GGC TC

58

105

[21]

Adamts1

F - CCA ACT GGA GCC ACA AAC ATT G

R - GGA CAG AGT GAA GTC GCC ATT C

55

126

[GenBank: XM_589626]

Adamts4

F - AAC TCG AAG CAA TGC ACT GGT

R - TGC CCG AAG CCA TTG TCT A

60

149

[44]

Adamts5

F - GCA TTG ACG CAT CCA AAC CC

R - CGT GGT AGG TCC AGC AAA CAG TTA C

55

97

[21]

Col2a1

F - TGA CCT GAC GCC CAT TCA TC

R - TTT CCT GTC TCT GCC TTG ACC C

55

154

[GenBank: X02420]

Mmp1

F - CAT TCT ACT GAC ATT GGG GCT CTG

R - TGA GTG GGA TTT TGG GAA GGT C

55

122

[GenBank: AF267156]

Mmp3

F - TCC CCC AGT TTC CCC TAA TG

R - GAT TTC TCC CCT CAG TGT GCT G

58

124

[GenBank: AF135232]

Mmp13

F - GGT GAC AGG CAG ACT TGA TGA TAA C

R - ATT TGG TCC AGG AGG GAA AGC G

58

349

[21]

Mmp14

R - CCC AGT GCT TGT CTC CTT TGA AG

56

126

[GenBank: AF267160]

Timp1

F - GGT TCA GTG CCT TGA GAG ATG C

R - GGG ATA GAT GAG CAG GGA AAC AC

57

265

[GenBank: S67450]

Timp2

F - ACT CTG GCA ACG ACA TCT ACG G

R - TCT TCT TCT GGG TGG CAC TCA G

57

261

[GenBank: M32303]

Timp3

F - CTT CCT TTG CCC TTC TCT ACC C

R - TCT GGT CAA CCC AAG CAT CG

57

286

[GenBank: NM_174473]

Gapdh

F - CCT GGA GAA ACC TGC CAA GTA TG

R - GGT AGA AGA GTG AGT GTC GCT GTT G

58

139

[GenBank: U94889]

Acan = aggrecan; Adamts = a disintegrin and metalloproteinase with thrombospondin motifs; F = forward primer; Gapdh = glyceraldehyde 3-phosphate dehydrogenase; Mmp = matrix metalloproteinase; R = reverse primer; Timp = tissue inhibitor of metalloproteinases.

Zreiqat et al. Arthritis Research & Therapy 2010 12:R16   doi:10.1186/ar2917