Research article
Flow cytometric characterization of freshly isolated and culture expanded human synovial cell populations in patients with chronic arthritis
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* Corresponding author: Rik J Lories Rik.Lories@uz.kuleuven.be
1 Laboratory for Skeletal Development and Joint Disorders, Division of Rheumatology, Katholieke Universiteit Leuven, Herestraat 49, Leuven, B3000, Belgium
2 Academic Unit of Musculoskeletal Disease, Leeds Institute of Molecular Medicine, University of Leeds, St James's University Hospital, Beckett Street, Leeds, LS9 7TF, UK
Arthritis Research & Therapy 2010, 12:R15 doi:10.1186/ar2916
Published: 27 January 2010Additional files
Additional file 1:
PDF file containing growth curves of cell cultures derived from (a) control and (b) inflamed synovium starting from passage one to six. ORT = cells derived from control samples; REU = cells derived from RA patients with active knee arthritis. Population doublings during each passage were calculated as the logarithm to two of the fold increase of cells (being harvested cells divided by seeded cells).
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Additional file 2:
PDF file containing plots showing the surface marker profile of cultured synovium-derived cells: representative sample. (a) Dot plots depicts the marker of interest in x-axis with 7-aminoactinomycin (7-AAD) staining in y-axis. (b) Dot plots depicting the controls used. The upper row shows the used isotype controls, with not much aspecific binding. The lower row shows cells only stained with 7-AAD ('fluorescence-minus-one' control).
Format: PDF Size: 936KB Download file
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