Figure 8.

CD16+ cells have a higher bone resorption activity than CD16^int cells. Sterile sorted MHCII+CD16+ and MHCII+CD16^int cells were co-cultured with bone slices in the presence of receptor activator of nuclear factor kappa-B ligand (RANKL) and macrophage colony-stimulating factor (M-CSF) for 14 days. (a) Quantification of bone pits eroded by MHCII+CD16^int and MHCII+CD16+ cells. (b) Reconstructed three-dimensional micro-CT images of the bone wafer incubated with MHCII+CD16^int [a] and MHCII+CD16+ [b] sorted cells. The data are representative of three independent sorting experiments.