Figure 6.

In vivo immune modulation by CD40 siRNA. a) and b) Inhibition of CII-specific antibody production in arthritis mice following CD40 siRNA. DBA mice were immunized with CII antigen and treated with CD40 siRNA or control siRNA, as described in Figure 3 or Figure 4. Sera were collected at the end point of experiments (five weeks after second immunization). The CII-specific antibodies from the mice in Figure 3 (A) and in Figure 4 (B) were detected by ELISA, as described in Materials and methods. c). Generation of Treg in arthritis mice following CD40 siRNA. DBA mice were immunized with CII antigen and treated with CD40 siRNA or control siRNA, as described in Figure 3. T cells were harvested from lymph nodes in the mice at the end point of experiments (five weeks after the second immunization). T cells were stained with mAbs against CD4, CD25 and Foxp3, respectively, and analyzed by flow cytometry. Results represent one of three experiments.

Zheng et al. Arthritis Research & Therapy 2010 12:R13   doi:10.1186/ar2914
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