Knockdown of CD40 reduces allogenicity in DCs. DBA-derived DCs were cultured as described in Materials and methods. DCs were transfected with CD40 siRNA using Genesilencer. Forty-eight hours after gene silencing, reduced gene expression of CD40 was detected by RT-PCR a) and flow cytometry b), respectively, without affecting CD80 gene expression determined by flow cytometry c). CD40-silenced DCs were also used to co-culture allogeneic (BALB/c) T cells. T cell proliferation was assessed in MLR d). Data are presented as mean ± SEM. Results represent one of three experiments. * = P < 0.05 versus control siRNA.
Zheng et al. Arthritis Research & Therapy 2010 12:R13 doi:10.1186/ar2914