Table 4

PCR sensitivities of the different DNA extraction methods detection Chlamydia trachomatis EB and C. trachomatis PBMO DNA/ml SF using PCR-system 1 for amplification immediately after extraction and post storage at -20°C for four months

Sensitivity achieved with PCR amplification system 1

1 Alkaline lysis immediately

1 Alkaline lysis ps

2 Qiaex II gel extraction kit^® +CTAB immediately

2 Qiaex II gel extraction kit^® + CTAB ps

4 Qiagen Tissue Kit^® immediately

4 Qiagen Tissue Kit^® Ps

5 Qiagen Stool Kit^® immediately

5Qiagen Stool Kit^® ps

(C. trachomatis EB/ml SF)

(0.1 -10)

M 1

10 (1-10)

M 10

0.1

1000 (1000)

M 1000

1 (1-10)

M 1

10 (1-10)

M 10

1 (1-100)

M 100

1 (1-10)

M 10

Statistical analysis ps

See Table 3

*

See Table 3

*

See Table 3

See Table 3

(C. trachomatis PBMO/ml SF)

(0.1-1)

M 0.1

1000 (1000)

M 1000

10 (0.1 - 100)

M 10

1000 (1000)

M 1000

10 (1-100)

M 10

1000 (1000)

M 1000

1 (0.1-1)

M 1

1000 (1000)

M 1000

Statistical analysis ps

See Table 4

*

See Table 4

*

See Table 4

*

See Table 4

*

Statistical analysis post storage (ps) compared with sensitivity results compared with sensitivity achieved after immediate PCR analysis are indicated by *. EB = elementary bodies; M = median; PBMO = peripheral blood monocytes; SF = synovial fluid.

Freise et al. Arthritis Research & Therapy 2009 11:R175   doi:10.1186/ar2864