Table 1 |
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Percentage of fibroblasts and contaminating cells in primary cultures of RA and OA synovial fibroblast cells at various passages |
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Passage |
Cell type |
% positive cells (Mean ± SEM)* |
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|
|
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RA SF |
OA SF |
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|
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|
First |
Fibroblast (Prolyl-4-hydroxylase +)1 |
75 ± 8.0 |
68 ± 5.0 |
|
Monocyte/macrophage (CD14+)2 |
15 ± 2.0 |
21 ± 3.5 |
|
|
T-cells (CD3+)3 |
0.8 ± 0.2 |
1.2 ± 0.3 |
|
|
B-cells (CD20+)4 |
0.9 ± 0.3 |
0.8 ± 0.2 |
|
|
Third |
Fibroblast (Prolyl-4-hydroxylase +) |
99 ± 0.5 |
98.5 ± 0.6 |
|
Monocyte/macrophage (CD14+) |
0.8 ± 0.2 |
0.6 ± 0.1 |
|
|
T-cells (CD3+) |
0.5 ± 0.1 |
0.8 ± 0.2 |
|
|
B-cells (CD20+) |
0.6 ± 0.2 |
0.5 ± 0.1 |
|
|
Fourth |
Fibroblast (Prolyl-4-hydroxylase +) |
98 ± 0.4 |
99.2 ± 0.4 |
|
Monocyte/macrophage (CD14+) |
1.0 ± 0.5 |
0.95 ± 0.3 |
|
|
T-cells (CD3+) |
0.5 ± 0.2 |
0.5 ± 0.1 |
|
|
B-cells (CD20+) |
0.9 ± 0.1 |
0.8 ± 0.1 |
|
|
|
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* Total number = five rheumatoid arthritis (RA) and five osteoarthritis (OA) patients. Monoclonal antibodies used for flow cytometry: mAb 5B5 1; mAb Tyk4 2; mAb UCHT-1 3; mAb B-Ly1 4. SEM = standard error of the mean; SF = synovial fluid. |
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Thwin et al. Arthritis Research & Therapy 2009 11:R138 doi:10.1186/ar2810 |
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