Table 1 |
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DNA sequences of the sense and antisense primers for real-time PCR analysis and cycler conditions |
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|
Gene |
Accession number |
Primer sequences |
Annealing temperature (°C) |
Number of cycles |
Product size (bp) |
|
|
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|
GAPDH |
[GenBank:J02642] |
5'-CAATGACCCCTTCATTGACC-3' (sense) 5'-TGGACTCCACGACGTACTCA-3' (antisense) |
59 |
30 |
197 |
|
IL-6 |
[GenBank:M14584] |
5'-CTTTTGGAGTTTGAGGTATACCTAG-3' (sense) 5'-CGCAGAATGAGATGAGTTGTC-3' (antisense) |
62 |
30 |
233 |
|
NFκB p65 |
[GenBank:NM_021975] |
5'-AGTACCTGCCAGATACAGACGAT-3' (sense) 5'-GATGGTGCTCAGGGATGACGTA-3' (antisense) |
62 |
30 |
215 |
|
Osteoprotegerin |
[GenBank:U94332] |
5'-TGCAGTACGTCAAGCAGGAG-3' (sense) 5'-CCCATCTGGACATCTTTTGC-3' (antisense) |
53 |
30 |
175 |
|
p27Kip1 |
[GenBank:NM_004064] |
5'-AGATGTCAAACGTGCGAGTG-3' (sense) 5'-TCTCTGCAGTGCTTCTCCAA-3' (antisense) |
59 |
40 |
154 |
|
RAGE |
[GenBank:AB036432] |
5'-GGAAAGGAGACCAAGTCCAA-3' (sense) 5'-CATCCAAGTGCCAGCTAAGA-3' (antisense) |
59 |
30 |
166 |
|
RANKL |
[GenBank:AF019047] |
5'-GCTTGAAGCTCAGCCTTTTG-3' (sense) 5'-CGAAAGCAAATGTTGGCATA-3' (antisense) |
59 |
40 |
192 |
|
TNF-α |
[GenBank:NM_000594] |
5'-GGCAGTCAGATCATCTTCTCGAA-3' (sense) 5'-AAGAGGACCTGGGAGTAGATGA-3' (antisense) |
62 |
40 |
195 |
|
|
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GAPDH = glyceraldehyde 3-phosphate dehydrogenase; NFκB = nuclear factor kappa B; RAGE = receptor for advanced glycation end products; RANKL = receptor activator of NFκB ligand. |
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Franke et al. Arthritis Research & Therapy 2009 11:R136 doi:10.1186/ar2807 |
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