Research article
Chondrogenic differentiation potential of osteoarthritic chondrocytes and their possible use in matrix-associated autologous chondrocyte transplantation
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* Corresponding author: Tilo Dehne tilo.dehne@charite.de
- † Equal contributors
1 Tissue Engineering Laboratory and Berlin-Brandenburg Center for Regenerative Therapies, Department of Rheumatology and Clinical Immunology, Charité-Universitätsmedizin Berlin, Tucholskystraße 2, Berlin, 10117, Germany
2 Institute of Laboratory Medicine, Department of Clinical Chemistry and Transfusion Medicine, Sahlgrenska University Hospital, Bruna Stråket 16, Gothenburg, SE 413-45, Sweden
Arthritis Research & Therapy 2009, 11:R133 doi:10.1186/ar2800
Published: 2 September 2009Additional files
Additional file 1:
Excel file containing a table of microarray expression data of comparative gene expression analysis. A total list of genes differentially expressed between cultures of chondrocytes obtained from osteoarthritic (OA) and normal donors (ND) is given. Group comparisons were performed between: scaffold and monolayer cultures from ND chondrocytes; scaffold and monolayer cultures from OA chondrocytes; chondrocytes from OA and ND cultured in monolayer; and scaffolds cultures (see to Figure 1 for experimental setup). Expression differences are given as fold change (FC) with monolayer or normal donor as baseline.
Format: XLS Size: 3.3MB Download file
This file can be viewed with: Microsoft Excel Viewer
Additional file 2:
Excel containing a table of microarray expression data of genes relevant for differentiation. Listed are genes that were differentially expressed in cultured chondrocytes obtained from osteoarthritic (OA) and normal donors (ND) resulting from following comparisons: scaffold and monolayer cultures from ND chondrocytes; scaffold and monolayer cultures from OA chondrocytes; chondrocytes from OA and ND cultured in monolayer; and scaffolds cultures. Genes were functionally filtered by annotations of the Gene Ontology Database (search terms 'skeletal development' and 'extracellular matrix formation'). Expression differences are given as fold change (FC). Either monolayer or ND was set as baseline.
Format: XLS Size: 207KB Download file
This file can be viewed with: Microsoft Excel Viewer