Table 2

Technical performance of the KS capture OA-1 ARGS ELISA

Linear measuring range of standard

0.02 to 1 pmol/ml

Minimal dilution of SF

1:50

Minimal detectable concentration in neat SF

1 pmol/ml SF

Intra assay CV (n = 10)

6.1%

*Inter assay, intra lot CV (n = 5, 1st lot)

12.2%

*Inter assay, intra lot CV (n = 23, 2nd lot)

15.6%

Inter assay, inter lot CV (n = 28)

19.7%


Dilution of SF

1:50

1:400

1:800

1:1600

Spiking recovery (mean; range)

116%;

109 to 121%

93%;

75 to 104%

81%;

75 to 88%

104%;

98 to 113%


* Between assay variation in lot numbers (1st, #5L21/1; 2nd, #7F25/1) of the KS capture plates from Biosource.

CV = coefficient of variation; KS = keratan sulfate; SF = synovial fluid.

Larsson et al. Arthritis Research & Therapy 2009 11:R92   doi:10.1186/ar2735

Open Data