Table 2 |
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|
Technical performance of the KS capture OA-1 ARGS ELISA |
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|
Linear measuring range of standard |
0.02 to 1 pmol/ml |
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|
Minimal dilution of SF |
1:50 |
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|
Minimal detectable concentration in neat SF |
1 pmol/ml SF |
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|
Intra assay CV (n = 10) |
6.1% |
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|
*Inter assay, intra lot CV (n = 5, 1st lot) |
12.2% |
|||
|
*Inter assay, intra lot CV (n = 23, 2nd lot) |
15.6% |
|||
|
Inter assay, inter lot CV (n = 28) |
19.7% |
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|
|
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|
Dilution of SF |
1:50 |
1:400 |
1:800 |
1:1600 |
|
Spiking recovery (mean; range) |
116%; 109 to 121% |
93%; 75 to 104% |
81%; 75 to 88% |
104%; 98 to 113% |
|
|
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|
* Between assay variation in lot numbers (1st, #5L21/1; 2nd, #7F25/1) of the KS capture plates from Biosource. CV = coefficient of variation; KS = keratan sulfate; SF = synovial fluid. |
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|
Larsson et al. Arthritis Research & Therapy 2009 11:R92 doi:10.1186/ar2735 |
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